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71.
Fluorescence spectroscopy of a green fluorescent protein mutant at single-molecule resolution has revealed a remarkable oscillatory behavior that can also be driven by applied fields. We show that immediately before unfolding, several periodic oscillations among the chemical substates of the protein chromophore occur. We also show that applied alternating electric or acoustic fields, when tuned to the protein characteristic frequencies, give rise to strong resonance effects. 相似文献
72.
A map of the interactome network of the metazoan C. elegans 总被引:1,自引:0,他引:1
Li S Armstrong CM Bertin N Ge H Milstein S Boxem M Vidalain PO Han JD Chesneau A Hao T Goldberg DS Li N Martinez M Rual JF Lamesch P Xu L Tewari M Wong SL Zhang LV Berriz GF Jacotot L Vaglio P Reboul J Hirozane-Kishikawa T Li Q Gabel HW Elewa A Baumgartner B Rose DJ Yu H Bosak S Sequerra R Fraser A Mango SE Saxton WM Strome S Van Den Heuvel S Piano F Vandenhaute J Sardet C Gerstein M Doucette-Stamm L Gunsalus KC Harper JW Cusick ME Roth FP Hill DE Vidal M 《Science (New York, N.Y.)》2004,303(5657):540-543
73.
Trinco Fabio Daniel Rusch Verónica Elena Howison Ruth Alison Garibaldi Lucas Alejandro Tittonell Pablo Adrián 《Agroforestry Systems》2021,95(8):1675-1688
Agroforestry Systems - Sound management of native forests used for cattle grazing requires understanding the dynamics of forage productivity in the openings. Despite their importance, forage... 相似文献
74.
da Silva Eder Eujácio Baio Fabio Henrique Rojo Teodoro Larissa Pereira Ribeiro Campos Cid Naudi Silva Plaster Octávio Barbosa Teodoro Paulo Eduardo 《Precision Agriculture》2022,23(1):35-51
Precision Agriculture - Defining the ideal soybean stand is essential for obtaining maximum grain yield. As a hypothesis of the work, it was questioned whether it is possible to vary seeding... 相似文献
75.
Jeann Leal de Araújo Alice C. A. M. Arruda Nayadjala T. A. Santos Glenison F. Dias Thiago F. L. Nery Fabio Del Piero Richard Ploeg Brian F. Porter Ingeborg M. Langohr 《Journal of veterinary diagnostic investigation》2021,33(3):600
A 4-mo-old northern red-shouldered macaw (Diopsittaca nobilis) was admitted to the veterinary hospital of the Arruda Câmara Zoo, in the State of Paraiba, Brazil, for investigation of an orbital mass. Given rapid progression and lack of response to treatment, the bird was euthanized, and an autopsy was performed. Histologically, the mass consisted of a retrobulbar invasive tumor characterized by tubular and rosette-like structures, with interspersed heteroplastic tissues, such as aggregates of neuroglial cells and islands of hyaline cartilage. The tumor was immunopositive for pancytokeratin, GFAP, NSE, and S100. These findings were compatible with an ocular teratoid medulloepithelioma, a neoplasm best described in humans but also reported rarely in young cockatiels and African Grey parrots. 相似文献
76.
Jessica K. Baron DVM DACVS-SA Sue A. Casale DVM DACVS Eric Monnet DVM PhD FAHA DACVS DECVS Philipp D. Mayhew BVM&S DACVS Jeffrey J. Runge DVM DACVS Christelle M. Follette DVM Kevin Phipps DVM Margaret E. Powell DVM Alicja I. Reczynska DVM Nathan T. Squire VMD Bruce A. Barton PhD John Berg DVM DACVS 《Veterinary surgery : VS》2020,49(Z1):O148-O155
77.
Salvatore Mastrangelo Slim Ben Jemaa Elena Ciani Gianluca Sottile Angelo Moscarelli Mekki Boussaha Marina Montedoro Fabio Pilla Martino Cassandro 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2020,137(6):609-621
The Valdostana is a local dual purpose cattle breed developed in Italy. Three populations are recognized within this breed, based on coat colour, production level, morphology and temperament: Valdostana Red Pied (VPR), Valdostana Black Pied (VPN) and Valdostana Chestnut (VCA). Here, we investigated putative genomic regions under selection among these three populations using the Bovine 50K SNP array by combining three different statistical methods based either on allele frequencies (FST) or extended haplotype homozygosity (iHS and Rsb). In total, 8, 5 and 8 chromosomes harbouring 13, 13 and 16 genomic regions potentially under selection were identified by at least two approaches in VPR, VPN and VCA, respectively. Most of these candidate regions were population-specific but we found one common genomic region spanning 2.38 Mb on BTA06 which either overlaps or is located close to runs of homozygosity islands detected in the three populations. This region included inter alia two well-known genes: KDR, a well-established coat colour gene, and CLOCK, which plays a central role in positive regulation of inflammatory response and in the regulation of the mammalian circadian rhythm. The other candidate regions identified harboured genes associated mainly with milk and meat traits as well as genes involved in immune response/inflammation or associated with behavioural traits. This last category of genes was mainly identified in VCA, which is selected for fighting ability. Overall, our results provide, for the first time, a glimpse into regions of the genome targeted by selection in Valdostana cattle. Finally, this study illustrates the relevance of using multiple complementary approaches to identify genomic regions putatively under selection in livestock. 相似文献
78.
79.
Irina GARCIA-ISPIERTO M. Angels ROSELLó Fabio DE RENSIS Fernando LóPEZ-GATIUS 《The Journal of reproduction and development》2013,59(6):544-548
This study compared the efficiency of a five-day or standard (nine-day)
progesterone-based regimen combined with equine chorionic gonadotrophin (eCG) in a
fixed-time AI (FTAI) protocol for dairy cows. The data examined were derived from
3577 inseminations conducted in three dairy herds. Animals with no estrus signs
detected over 21 days were randomly assigned to a PRID-9 or PRID-5 group. Cows in
each group received a progesterone intravaginal device (PRID) for 9 or 5 days,
respectively, PGF2α and eCG on PRID removal, and GnRH 48 h later.
Fixed-time AI was performed 12 h after the GnRH dose. Cows artificially inseminated
following spontaneous estrus during the study period were considered as controls.
Based on the odds ratio, the likelihoods of animals in PRID-9 in the warm (conception
rate [CR] of 22.3%) and cool (32% CR) periods, and control animals in the warm period
(26.6% CR) becoming pregnant were reduced (by factors of 0.6, 0.3 and 0.4,
respectively) compared with the control animals in the cool period (CR of 43.7%). The
risk of a twin pregnancy was higher (51.4%) for cystic PRID-9 cows (by a factor of
3.6) and lower (9.9%) for cyclic PRID-5 animals (by a factor of 0.4) compared with
the PRID-9 cyclic cows. These findings indicate that the proposed protocol achieves
similar results during the cool or warm season to those obtained when AI is conducted
at spontaneous estrus during the cool season. In addition, PRID-5 reduced twin
pregnancy compared with PRID-9. 相似文献
80.
Simone M. Cacciò Anna Rosa Sannella Valeria Mariano Silvia Valentini Franco Berti Fabio Tosini Edoardo Pozio 《Veterinary parasitology》2013,191(1-2):128-131
An outbreak of cryptosporidiosis occurred in a mixed sheep/cattle farm of Central Italy in October 2011. A total of 450 ovines (250 sheep and 200 lambs) and 140 bovines (130 cows and 10 calves) were housed in two separated units, at the time of the outbreak. About half of the lambs had diarrhea due to Cryptosporidium sp. with a mortality rate of 80%; calves were not infected. Genomic DNA was extracted from an archived slide and from fecal specimens, and the parasite was identified as Cryptosporidium parvum by PCR and sequence analysis at the CpA135 gene. Genotyping at the GP60 gene showed the presence of a very rare genotype, IIaA20G2R1. Shortly after the outbreak was identified, the son of the farm's owner, aged 18 months, experienced an acute gastroenteritis and was hospitalized due to recurrent episodes of diarrhea, fever, vomiting and lack of appetite. The feces tested negative for bacteria and viruses, whereas cryptosporidiosis was diagnosed by microscopy and an immunochromatographic test. Molecular typing identified the C. parvum genotype IIaA20G2R1 in the feces of the child. This is the first case of transmission of cryptosporidiosis in Italy involving lambs as source of oocysts infectious to humans. 相似文献